phospho src tyr416 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc phospho src tyr416
Phospho Src Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+src+tyr416/Phospho-Src+Family+(Tyr416)+Antibody/us12594421-308-66-69
Average 96 stars, based on 2354 article reviews

phospho src tyr416 - by Bioz Stars, 2026-09

96/100 stars

Images

other:

Article Title: ATT-Myc Transgenic Mouse Model and Gene Expression Identify Genotoxic and Non-Genotoxic Chemicals That Accelerating Liver Tumor Growth in Short-Term Toxicity
Article Snippet: Phospho-Src (Tyr416) , Cell Signaling , 1:1000 , 2101.

Electrophoresis:

Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

Membrane:

Western Blot:

Article Title: Mechanistic Exploration of Shugan Jianpi Formula for Treating Triple-Negative Breast Cancer Under Chronic Stress: A Network Pharmacology-Guided Experimental Study
Article Snippet: Protein bands were detected using the ECL reagent and analyzed with a chemiluminescence Western blot detection system (Tanon, China). .. Phospho-Src (Tyr416) (CST; cat. #6943), Src (CST; cat. #2109), Phospho-Stat3 (Tyr705) (CST; cat. #9145), Stat3 (CST; cat. #9139), p44/42 mitogen-activated protein kinase (MAPK) (Erk1/2) (CST; cat. #4695), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (CST; cat. #4370), Plk1 (208G4) (CST; cat. #4513), Skp2 (CST; cat. #2652), VEGFA (Proteintech; cat. #19003-1-AP), Vimentin (Proteintech; cat. #10366-1-AP), E-Cadherin (Proteintech; cat. #20874-1-AP), N-Cadherin (Proteintech; cat. #22018-1-AP), Bax (Proteintech; cat. #50599-2-Ig), Bcl2 (Proteintech; cat. #68103-1-Ig), Caspase-3 (CST; cat. #9662), Caspase-9 (CST; cat. #9502),Vinculin (CST; cat. #66305-1-Ig) were used for Western blot. ..

Article Title: PEAK1 maintains tight junctions in intestinal epithelial cells and resists colitis by inhibiting autophagy-mediated ZO-1 degradation.
Article Snippet: Antibodies against the following proteins were purchased from Invitrogen: ZO-1 Alexa FluorTM 555 (IF 1:100, MA3-39100-A555), Claudin-2 (WB 1:1,000, IF 1:100, 32-5600), Goat anti-Mouse IgG (H + L) Alexa Fluor® 488 Secondary Antibody (1:500, A-11029), Goat antiRabbit IgG (H+ L) Alexa Fluor® 488 Secondary Antibody (1:500, A11034), Goat anti-Mouse IgG (H + L) Alexa Fluor® 555 Secondary Antibody (1:500, A-21424), Goat anti-Rabbit IgG (H + L) Alexa Fluor® 555 Secondary Antibody (1:500, A-21429). .. Antibodies against the following proteins were purchased from Cell Signaling Technology: Src (WB 1:1,000, #2108), Phospho-Src (Tyr416) (1:1,000, #2101), Phospho-Src (Tyr527) (WB 1:1,000, #2105), ERK1/2 (WB 1:1,000, #4695), LC-3B (WB 1:1,000, #2775), Phospho-Myosin Light Chain 2 (Ser19) (WB 1:1,000, #3671), Myosin Light Chain 2 (WB 1:1,000, #8505), Conformation Specific Mouse Anti-Rabbit IgG (WB 1:2,000, #5127S). .. Antibodies against the following proteins were purchased from Bioworld: Occludin (WB 1:1,000, AP0765), GFP (WB 1:2,000, MB9233), and E-cadherin (WB 1:1,000, BS1098).

Incubation:

Article Title: Omentin-1 attenuates atrial fibrillation via Src/PI3K/Akt signaling-mediated anti-fibrotic effects in cardiac fibroblasts.
Article Snippet: Background: Atrial fibrillation (AF) is characterized by progressive atrial fibrosis, leading to increased morbidity and mortality.. While the novel adipokine Omentin-1 demonstrates anti-fibrotic potential across organ systems, its role in AF pathogenesis remains unclear.. This study investigates Omentin-1’s therapeutic effects and the underlying mechanisms in angiotensin II (Ang II)-induced atrial fibrosis and AF.

Article Title: Modulation of blood-tumor barrier transcriptional programs improves intratumoral drug delivery and potentiates chemotherapy in GBM.
Article Snippet: Blocking was performed in 5% milk with 0.1% Triton X- 100 in 1× PBS (TBST) (Gibco) for 1 hour at room temperature under shaking. .. Primary antibodies used were incubated in the cold under shaking overnight: antipCHK1 (Ser345) (1:100; Cell Signaling Technology), anti- CHK1 (1:1000; Cell Signaling Technology), anti- pH2AX (Ser139) (1:1000; Cell Signaling Technology), anti- WNT7A/B (1:1000; Proteintech), anti–IL- 6 (1:1000; Thermo Fisher Scientific), anti–phospho- GSK3b (Ser9) (1:1000; Cell Signaling Technology), anti- vinculin (1:1000; Proteintech), anti–phospho- TYK2 (Tyr1054 and Tyr1055) (1:1000; Thermo Fisher Scientific), anti- phospho- STAT5A/B (Tyr694) (1:1000; Cell Signaling Technology), anti–phospho- p38α (Thr180 and Tyr182) (1:1000; Thermo Fisher Scientific), anti–phospho- SRC (Tyr416) (1:1000; Cell Signaling Technology), anti–phospho- CREB (Ser133) (1:500; Thermo Fisher Scientific), anti- MFSD2A (1:500; Proteintech), anti- CAV1 (1:1000; Proteintech), anti- CD144 (VE- cadherin) (1:1000; Thermo Fisher Scientific), and anti–β- actin (1:2000; Cell Signaling Technology). .. The appropriate secondary antibody goat anti–mouse– horseradish peroxidase (HRP; Sigma- Aldrich) or goat anti–rabbitHRP (Sigma- Aldrich) was used in 5% milk in 1× TBST with 1:5000 dilution for 1 hour at room temperature.

Review